Nitrate and nitrite are notoriously hard to quantify when in solution together. In many cases, this doesn’t matter as nitrite is only present in small amounts and the sum of the two is sufficient for process control. In wastewater treatment processes, however, being able to separately monitor nitrite from nitrate is crucial.
Standard wastewater treatment processes use oxygen and nitrifying bacteria to remove ammonia to nitrogen gas via nitrite and nitrate. In such cases, the amount of nitrite present at any given time is relatively low.
Reaction route of conventional Nitrification/Denitrification
In treatment processes, the aim is to reduce the amount of energy used and the process is shortened by the partial nitritation of ammonia to nitrite and it’s subsequent denitritation directly to nitrogen gas.
Denitrification using Anammox method
In such processes the measurement of nitrite alone becomes critical, firstly to ensure the nitritation process is working correctly but secondly to ensure that little nitrate is being produced.
From an analytical instrumentation perspective, this poses an issue. There are two main methods of determining nitrate and nitrite, one is colorimetric and the other uses UV absorbance.
In treatment processes, the aim is to reduce the amount of energy used and the process is shortened by the partial nitritation of ammonia to nitrite and it’s subsequent denitritation directly to nitrogen gas.
UV absorbance methods for nitrate and nitrite use the fact that both nitrogen containing compounds absorb UV light between 210 and 220nm. In most traditional wastewater treatment processes, separating the response from nitrate and nitrite is not important because nitrate is the predominant form and you want to measure the degree of nitrification. Additionally, the contribution of nitrite to the UV absorbance in this region is relatively low compared to nitrate, so most manufacturers of analytical instrumentation simply report the result as NO3 and include the NO2 (and assume the contribution from NO2 is small). In wastewater treatment processe this isn’t good enough. Such processes need accurate measurement of nitrite, if any nitrate does occur, then it indicates something isn’t working correctly in the process. Additionally, if you’re not separating the response from nitrate and nitrite, as soon as any nitrate is present, it’s contribution to the UV absorbance overwhelms the response from nitrite.
1: <1nm resolution
The UV only spectro::lyser takes 256 absorbance measurements across the UV region (190-390nm). This means it can take measurements at approximately every 1nm, enabling it to separate out the response from nitrate at 220nm, from nitrite at 210nm.
2: Flexible path length installation and solids compensation algorithms.
s::can spectro::lysers can be installed with very small path lengths (0.5 or 1mm) and have a unique way of reducing the impact on absorbance from suspended solids. By measuring multiple wavelengths and building a model of how solids are contributing to the absorbance, it can filter out the effect ensuring that the response to nitrate and nitrite is accurate even in the highly turbid samples that may be seen in the processes. Using a small path length also ensures the sensor doesn’t get ‘blinded’ by high suspended solids.
